• Pluripotency tests included flow cytometry analysis of prote

  2018-10-24

  

  Pluripotency tests included flow cytometry analysis of protein stem cell markers SSEA3, SSEA4, Tra1-60, Tra1-81 and Oct3/4 (Fig. 2A), alkaline phosphatase staining (ALP) (Fig. 2B), and qRT-PCR analysis of endogenous pluripotent transcription factors OCT-4, SOX-2, NANOG and REX1 (Fig. 2C). To finally

• br Materials and methods br Author disclosure statement

  2018-10-24

  

  Materials and methods Author disclosure statement Acknowledgments We thank Muscle Tissue Culture Collection (MTCC) for providing the sample. The Muscle Tissue Culture Collection is part of the German network on muscular dystrophies (MD-NET, service structure S1, 01GM0601) and the German net

• br Introduction Capable of almost

  2018-10-24

  

  Introduction Capable of almost unlimited proliferation and efficient differentiation into cardiomyocytes (Yang et al., 2008; Lian et al., 2012; Elliott et al., 2011), hPSCs open a new route for the research of human buy betaxolol development and heart diseases, drug development, and most importa

• Intestinal epithelial SCs IESCs are roughly categorized as e

  2018-10-24

  

  Intestinal epithelial SCs (IESCs) are roughly categorized as either quiescent or active IESCs based in part on the expression of specific markers, including LGR5, Olfm4, Ascl2, BMI1, MTERT, and LRIG1 (Barker et al., 2007; Montgomery et al., 2011; Powell et al., 2012; Sangiorgi and Capecchi, 2008). T

• iPSC derived iHLCs offer a

  2018-10-24

  

  iPSC-derived iHLCs offer a unique advantage in that they can be generated from any donor, which allows a broad spectrum of the human population to be represented in in vitro drug screens and provides an opportunity to assess donor-specific drug responses in vitro. Recent collaborations between drug

• Compared to treatments with other FGF isoforms

  2018-10-24

  

  Compared to treatments with other FGF2 isoforms, stimulation with 22.5kDa FGF2 results in lower stem cell gene expression in hESCs (Fig. 4d), weaker proliferative activity in human dermal fibroblasts (Fig. 5), and lower relative mitogenic activity in BaF3 sphk ectopically expressing FGFR1 IIIb, one

• An increase in the PGC number caused by dnd

  2018-10-24

  

  An increase in the PGC number caused by dnd RNA injection does not exceed 3-fold, even if a high level of Dnd expression occurs in many cells. It is likely that an additional factor essential for PGC specification exists in medaka, which shows expression and PGC-specifying activity similar to dnd bu

• These data are in agreement with prior studies

  2018-10-24

  

  These data are in agreement with prior studies demonstrating that hyaluronan levels play a critical role in determining the site of HSC lodgment/engraftment following transplantation (Avigdor et al., 2004; Ellis et al., 2007, 2009, 2011; Nilsson et al., 2003), and that HSC exhibit preferential engra

• A variety of studies have compared the expression profiles

  2018-10-24

  

  A variety of studies have compared the expression profiles, pluripotentiality, and genetic and epigenetic stability of hESC and iPSC including lines generated using different strategies, distinct parental somatic cell types, or reprogramming methods (Bock et al., 2011; International Stem Cell Initia

• To determine whether the inhibition of

  2018-10-24

  

  To determine whether the inhibition of RSK and TTK inhibits neovascularization in vivo, we gave mice LL/2 tumor grafts. Both BI-D1870 and AZ3146, used at doses determined to be non-toxic to animals, significantly improved survival, inhibited tumor growth, and decreased vascular density. In contrast,

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments We appreciate the expert technical assistance of N. Hayashi, K. Iseki, T. Hashiguchi, W. Ohashi, M. Kawahara, S. Okamoto, and C. Yamada (Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe). We appre

• br Results br Discussion We

  2018-10-24

  

  Results Discussion We report the development of a method to generate and proliferate iCx26GJCs from mouse iPSCs for use as a disease model and in inner-ear cell therapies targeting GJB2-related hearing loss, the most frequent type of hereditary deafness worldwide. Our induction method until da

• thymidylate synthase inhibitors We functionally screened spo

  2018-10-24

  

  We functionally screened spontaneously active CMs for pacemaker properties, revealing that the majority of such thymidylate synthase inhibitors had ventricular- or atrial-like but not nodal-like properties, when APs were elicited from physiological resting potentials (−80 mV). Nav1.5 is the main so

• A complementary study confirms our findings that GATA is nec

  2018-10-24

  

  A complementary study confirms our findings that GATA6 is necessary for phosphodiesterase inhibitors specification as well as the decreased efficiency of β-like cell generation from the GATA6 PSC line (Shi et al., 2017). This other report did have one major discrepancy with our results; they did no

• A nuance of the conventional conditions has been that while

  2018-10-24

  

  A nuance of the conventional conditions has been that while overall amplification of cell number occurs in the cultures, the percentage of the population possessing regenerative capacity (i.e., SSCs) declines over a several-month period (Kanatsu-Shinohara et al., 2003, 2005, 2011; Kubota et al., 200

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