Further support for the hierarchical two

2018-10-20

Further support for the hierarchical two-stem-cell model came with the discovery of an additional reserve ISC marker locus, Hopx, encoding an atypical homeodomain protein with functions in early heart development (Chen et al., 2002). An IRES-CreERT2 cassette inserted into the endogenous Hopx locus r

Primary human fibroblast cultures were

2018-10-20

Primary human fibroblast cultures were obtained from skin biopsies of FTD patients after informed consent; control iPSCs were generated from BJ fibroblasts. Human iPSC lines were generated and characterized as previously described (Takahashi et al., 2007b). hESC and iPSC lines were differentiated to

TH-302 Our study shows that iPSCs are highly proficient in a

2018-10-20

Our study shows that iPSCs are highly proficient in antioxidant defense, which is presumably responsible for the low frequency of oxidative DNA lesions in both the mitochondrial and nuclear genome. Notably, previous non-quantitative proteomic studies revealed an abundant expression of antioxidant en

Alternative approaches to LV are non viral vectors

2018-10-20

Alternative approaches to LV are non-viral vectors such as RNA or DNA, which are considered safer due to their integration-free nature (Hardee et al., 2017). However, manufacturing RNA is expensive and difficult because RNA has a propensity to degrade (Yin et al., 2014). On the other hand, DNA plasm

melanocortin receptor To trace host to graft connectivity of

2018-10-20

To trace host-to-graft connectivity of transplanted hESC-derived neurons, we next generated hESC lines that stably expressed the tracing and control constructs (Figures 2A and S2A). The engineered hESCs were differentiated using a protocol that results in a high yield of authentic and functional ven

To determine whether the inhibition of RSK and

2018-10-20

To determine whether the inhibition of RSK and TTK inhibits neovascularization in vivo, we gave mice LL/2 tumor grafts. Both BI-D1870 and AZ3146, used at doses determined to be non-toxic to animals, significantly improved survival, inhibited tumor growth, and decreased vascular density. In contrast,

br Results To investigate the molecular and

2018-10-20

Results To investigate the molecular and cellular phenotypes perturbed in DS, we compared DS and normal ESCs and their neural derivatives. In the past, we have isolated three DS-ESC lines, namely CSES13, CSES20, and CSES21, from PGS-derived embryos with trisomy of chromosome 21 (Figure 1A) (Bianc

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Introduction Frontotemporal dementia (FTD) refers to a group of neurodegenerative diseases caused by focal but progressive neuronal loss, astrogliosis, and spongiosis in the frontal and temporal cortices associated with abnormal intracellular acc

The correct deposition of m C

2018-10-20

The correct deposition of m5C into RNAs is essential for normal development. Loss-of-function mutations in the NSUN2 gene in both mouse and human cause growth retardation and neurodevelopmental deficits including microcephaly, as well as defects in cognition and motor function (Blanco and Frye, 2014

Hello world!

2018-07-29