• Recombinant Mouse Sonic Hedgehog Protein in Genital Tuber...

  2025-09-22

  Explore the applications of Recombinant Mouse Sonic Hedgehog (SHH) Protein in elucidating mammalian genital tubercle, urethral groove, and prepuce patterning. This article highlights SHH's mechanistic roles, experimental approaches, and translational relevance for developmental biology and congenital malformation research.

• ARCA Cy5 EGFP mRNA (5-moUTP): Precision Tools for Dissect...

  2025-09-19

  Explore the application of ARCA Cy5 EGFP mRNA (5-moUTP) as a 5-methoxyuridine modified, fluorescently labeled mRNA for delivery analysis and translation efficiency assays in mammalian cells. This article provides a rigorous overview of its utility in studying mRNA localization, innate immune suppression, and optimization of mRNA-based reporter systems.

• Z-VAD-FMK: Dissecting Apoptotic Pathways in RNA Pol II-Tr...

  2025-09-18

  Explore how Z-VAD-FMK, a cell-permeable pan-caspase inhibitor, illuminates the mechanistic links between RNA Pol II inhibition and apoptosis. This article provides a rigorous analysis of its applications in studying apoptotic signaling and caspase activity in advanced cell death models.

• As one of emerging high throughout methods metabolomics dete

  2025-03-03

  

  As one of emerging high-throughout methods, metabolomics detects metabolites which are ultimate response of biological systems to environmental changes [14]. Metabolomics uncovers metabolic alteration at the systems level. Comparison of global metabolic profiling reflects some unidentified biologic

• ACK has been clearly implicated in cancer progression in

  2025-03-03

  

  ACK1 has been clearly implicated in cancer progression in recent years. A strong correlation between ACK1 gene copy number, protein level and activity have been demonstrated in tumors of different tissue types (Mahajan et al., 2007; van der Horst et al., 2005; Wang et al., 2006). Our western blot an

• br Perspectives Given the complexity of

  2025-03-03

  

  Perspectives Given the complexity of the cellular interactome and of protein scaffolding, further progress in revealing how individual membrane proteins, including APP and AChE, interact with each other at various levels, from cell-surface shedding to gene regulation, will clarify some intrinsic

• The observation that vortioxetine blocks

  2025-03-03

  

  The observation that vortioxetine blocks 5-HT-induced currents in 5-HT3 receptor-expressing oocytes suggests that vortioxetine acts to functionally antagonize these receptors, and is in line with previously published data (Bang-Andersen et al., 2011). For example, Mørk et al. (2012) demonstrated tha

• In this scenario the receptor tyrosine kinase inhibitors

  2025-03-03

  

  In this scenario, the receptor tyrosine kinase inhibitors (TKI), sunitinib, an anti-angiogenic agent, was tested in phase III trial, leading to a longer progressing free survival when compared to interferon (median: 11 vs. 5mo, respectively, hazard ratio (HR) = 0.42; 95% CI: 0.32–0.54; Pits Arylcycl

• IL-2, human recombinant Our results showed suppressed autoph

  2025-03-03

  

  Our results showed suppressed autophagy in GECs under HAGG stimulation. Recently, people have paid more attentions on the roles of autophagy in lupus and tried to use autophagy regulators for therapy. Rapamycin could prevent the development of nephritis [49] and attenuate the established nephritis [

• Purification of Rv c In order to investigate

  2025-03-03

  

  Purification of Rv2477c. In order to investigate the catalytic capabilities of Rv2477c, we expressed the protein as an N-terminal histidine-tagged fusion protein in E. coli BL21 cells and purified the protein by cobalt-affinity chromatography. As shown in Fig. 2A, the 65 kDa fusion protein was expre

• To clarify the molecular mechanism of

  2025-03-01

  

  To clarify the molecular mechanism of maduramicin-induced apoptosis in chicken myocardial cells, the mRNA levels of bcl-2, bax, and cytochrome C, as well as the mRNA levels and activities of caspase-3/8/9 were determined. The mRNA expressions and activities of initiator casapase-9 and executioner ca

• In Antirrhinum floral organ identity genes include DEFICIENS

  2025-03-01

  

  In Antirrhinum, floral organ identity genes include DEFICIENS (DEF) (Sommer et al., 1990) and GLOBOSA (GLO) (Trobner et al., 1992) required for petal and stamen identity (B-function), PLENA (P) required for stamen and carpel formation (C-function) (Bradley et al., 1993) and SQUAMOSA (SQUA) which is

• Introduction Heterotrimeric G proteins mediate signal transd

  2025-03-01

  

  Introduction Heterotrimeric Gαβγ proteins mediate signal transduction through seven-transmembrane domain receptors. In response to neurotransmitters or hormones, activated receptors bind GTP via the Gα subunit leading to the dissociation of Gα from the Gβγ dimer. G proteins regulate the levels of s

• It remains unclear to what extent UCP and UCP are

  2025-03-01

  

  It remains unclear to what extent UCP2 and UCP3 are subject to the same acute molecular regulation as UCP1 (and the extent to which they share the same mechanism of uncoupling). Although they lack sequence homology in a matrix-localised region reportedly critical for fatty proton pump inhibitors lis

• At Johns Hopkins Aramco Health JHAH we started an ASP

  2025-03-01

  

  At Johns Hopkins Aramco Health (JHAH), we started an ASP in 2011 with an educational program of physicians and pharmacist. The program was enhanced mid-2012 with the following interventions: a re-designed antibiotic sensitivity report [2], intravenous to oral conversion program, vancomycin pharmacok

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